Journal
BIOMATERIALS
Volume 27, Issue 3, Pages 371-376Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2005.06.032
Keywords
disc; tissue engineering; proteoglycans; extracellular matrix
Ask authors/readers for more resources
The objective of the present study was to assess proteoglycan production by human intervertebral disc cells cultured in vitro in selected cell carriers. Based on previous studies which evaluated disc cells seeded into collagen sponge, collagen gel, agarose, alginate or fibrin gel three-dimensional (3D) cell carriers, collagen sponge and agarose were found to provide superior microenvironments for formation of extracellular matrix (ECM). A standardized test design was used to evaluate ECM formed after 14 days of culture using the 1,9-dimethylmethylene blue (DMB) assay to assess sulfated glycosaminoglycan (S-GAG) production. Although agarose culture showed higher S-GAG levels compared to collagen sponge (2.94 +/- 2.20 (19)mu g/ml S-GAG (mean +/- S.D. (n)) vs. 0.94 +/- 0.77 (22), respectively, p = 0.0003), this is off-set by the significantly lower proliferation rate associated with culture of disc cells in agarose. (c) 2005 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available