Journal
FOOD CHEMISTRY
Volume 94, Issue 2, Pages 226-231Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2004.11.008
Keywords
anthocyanins; Smilax aspera; HPLC-DAD; mass spectrometry; natural colorants
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Anthocyanins, present in the berries of Smilax aspera L., a creeping shrub typical of the Mediterranean region, were extracted and identified for the first time. The pigments were extracted from the skin of the berries with of 0.1% HCl in methanol solution, purified on a C-18 solid-phase cartridge and characterized by means of HPLC-DAD-MS analysis. From spectral analysis before and after acid and alkaline hydrolysis, it was noted that pelargonidin 3-O-rutinoside represented about 83% of the total anthocyanin content in the skin of Smilax aspera berries. A low quantity of cyanidin 3-O-rutinoside (13%) was also found. The attractive colour and the great abundance of the plant in the south of Italy make Smilax aspera berries a new and very good source of natural pigments. (c) 2004 Elsevier Ltd. All rights reserved.
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