Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 70, Issue 1, Pages 296-299Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.70.296
Keywords
deep-sea yeast; Cryptococcus liquefaciens strain N6; polygalacturonase; high hydrostatic pressure
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A deep-sea yeast, Cryptococcus liquefaciens strain N6, produces two polygalacturonases, p36 and p40 (N6-PGases). These N6-PGases were highly active at 0-10 degrees C in comparison to a PGase from Aspergillus japonicus. The hydrolytic activity of these N6-PGases remained almost unchanged up to a hydrostatic pressure of 100 MPa at 24 degrees C with a very small activation volume of -1.1ml/mol. At 10 degrees C, however, the activation volume increased to 3.3 or 5.4ml/mol (p36 and p40, respectively), suggesting that the enzyme-substrate complexes can expand at their transition states. We speculate that such a volume expansion upon forming the enzyme-substrate complexes contributes to decreasing the activation energy for hydrolysis. This can account for the high activity of N6-PGases at low-temperature.
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