Analysis of organochlorine pesticides in wine by solvent bar microextraction coupled with gas chromatography with tandem mass spectrometric detection

A solvent bar microextraction (SBME) technique combined with gas chromatography/tandem mass spectrometry (GC/MS/M4S), for the determination of selected organochlorine pesticides (OCPs) in wine samples, is described. In this work the OCPs were extracted and dissolved in a 2-mu L aliquot of organic extraction solvent (n-tetradecane) confined within a 1.7-cm length of hollow fiber. Both ends of the hollow fiber (solvent bar) were sealed, and it was placed in an aqueous sample solution for extraction. The effects of solvent selection, sample agitation, extraction time, extraction temperature, and salt concentration on the SBME performance were optimized. The influence of aqueous sample/organic solvent phase ratio was further investigated in detail. High enrichments (1900-7100-fold) could be obtained at an aqueous sample/organic solvent volume ratio of 20 mL/2 mu L in this study. Good extraction repeatabilities were obtained with relative standard deviation (RSD) values below 12.6%. Comparisons of sensitivity and precision between SBME and dynamic hollow-fiber liquid-phase microextraction were also investigated. Copyright (c) 2005 John Wiley & Sons, Ltd.