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Methanosaeta harundinacea sp nov., a novel acetate-scavenging methanogen isolated from a UASB reactor

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MICROBIOLOGY SOC
DOI: 10.1099/ijs.0.63887-0

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Two methanogenic strains, 8Ac(T) and 6Ac, were isolated from an upflow anaerobic sludge blanket reactor treating beer-manufacture wastewater in Beijing, China. Cells of strains 8Ac(T) and 6Ac were rod-shaped (0.8-1.0 x 3.5 mu m) and non-motile, occurring singly or in pairs; however, at high cell density the cells were arranged in long chains within a common sheath. The two strains used acetate exclusively for growth and methane production. The specific growth rate of strain 8Ac(T) was 0.030 h(-1) when growing in acetate (20 mM) at 37 degrees C. The temperature range for growth was 25-45 degrees C, with the fastest growth at 34-37 degrees C. The pH range for growth and methane production was 6.5-9.0, with the fastest growth at pH 7.2-7.6. The G+C content of genomic DNA of strain 8Ac(T) was 55.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the novel strains clustered with Methanosaeta species; the 16S rRNA gene sequence similarities between strain 8Ac(T) and Methanosaeta concilii DSM 3013 and 'Methanosaeta thermophila' DSM 6194 were 92.5 and 87.3%, respectively. The sequence similarity levels of mcrA, the gene encoding the alpha-subunit of methyl-coenzyme M reductase, and of the deduced amino acids of mcrA, between strain 8Ac(T) and Methanosaeta concilii DSM 3671(T) were 36 and 78.9%, respectively. Based on the phylogenetic and phenotypic analyses, the novel species Methanosaeta harundinacea sp. nov. is proposed, with strain 8Ac(T) (= JCM 13211(T) = CGMCC 1.5026(T)) as the type strain.

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