Journal
CLINICA CHIMICA ACTA
Volume 363, Issue 1-2, Pages 106-119Publisher
ELSEVIER
DOI: 10.1016/j.cccn.2005.05.041
Keywords
microbial diagnostics; microarray; hybridization; oligonucleotide; marker gene
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Oligonucleotide microarrays are highly parallel hybridization platforms, allowing rapid and simultaneous identification of many different microorganisms and viruses in a single assay. in the past few years, researchers have been confronted. with a dramatic increase in the number of studies reporting development and/or improvement of oligonucleotide microarrays for microbial diagnostics, but use of the technology in routine diagnostics is still constrained by a variety of factors. Careful development of microarray essentials (such as oligonucleotide probes, protocols for target preparation and hybridization, etc.) combined with extensive performance testing are thus mandatory requirements for the maturation of diagnostic microarrays from fancy technological gimmicks to robust and routinely applicable tools. (c) 2005 Elsevier B.V. All rights reserved.
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