Journal
CLINICA CHIMICA ACTA
Volume 363, Issue 1-2, Pages 48-60Publisher
ELSEVIER
DOI: 10.1016/j.cccn.2005.04.037
Keywords
nucleic acid hybridization; real-time gene amplification assays; fluorescence energy transfer; molecular beacons; fluorescent nucleic acid hybridization probes
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U01AI056689] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB000277] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM070357] Funding Source: NIH RePORTER
- NIAID NIH HHS [AI-056689] Funding Source: Medline
- NIBIB NIH HHS [EB-000277] Funding Source: Medline
- NIGMS NIH HHS [GM-070357] Funding Source: Medline
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Background: A number of formats for nucleic acid hybridization have been developed to identify DNA and RNA sequences that are involved in cellular processes and that aid in the diagnosis of genetic and infectious diseases. Methods: The introduction of hybridization probes with interactive fluorophore pairs has enabled the development of homogeneous hybridization assays for the direct identification of nucleic acids. A change in the fluorescence of these probes indicates the presence of a target nucleic acid, and there is no need to separate unbound probes from hybridized probes. Conclusions: The advantages of homogeneous hybridization assays are their speed and simplicity. In addition, homogeneous assays can be combined with nucleic acid amplification, enabling the detection of rare target nucleic acids. These assays can be followed in real time, providing quantitative determination of target nucleic acids over a broad range of concentrations. (c) 2005 Elsevier B.V. All rights reserved.
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