Journal
FEMS MICROBIOLOGY LETTERS
Volume 254, Issue 2, Pages 251-257Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2005.00029.x
Keywords
Tuber melanosporum; soil mycelium; PCR-RFLP; truffle orchard; ITS
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Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T melanosporum. The detection limit was >= 11.4 mu g of hyphae g(-1) of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.
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