Journal
NATURE PROTOCOLS
Volume 1, Issue 6, Pages 2733-2741Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2006.229
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Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM036326] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM036326, GM-36326, R01 GM036326-19, R01 GM036326-13] Funding Source: Medline
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Circular dichroism (CD) is an excellent tool for examining the interactions and stability of proteins. This protocol covers methods to obtain and analyze circular dichroism spectra to measure changes in the folding of proteins as a function of denaturants, osmolytes or ligands. Applications include determination of the free energy of folding of a protein, the effects of mutations on protein stability and the estimation of binding constants for the interactions of proteins with other proteins, DNA or ligands, such as substrates or inhibitors. The experiments require 2-5 h.
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