Journal
NATURE PROTOCOLS
Volume 1, Issue 1, Pages 436-443Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2006.64
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Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM073042] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM073042] Funding Source: Medline
- PHS HHS [NIGMS 60642, NIGMS 73042] Funding Source: Medline
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The value of recognizing cellular RNA sequences by short interfering RNAs (siRNAs) in mammalian cells is widely appreciated, but what might be learned if it were also possible to recognize chromosomal DNA? Recognition of chromosomal DNA would have many applications, such as inhibiting gene expression, activating gene expression, introducing mutations, and probing chromosome structure and function. We have shown that antigene peptide nucleic acids (agPNAs) and antigene duplex RNAs (agRNAs) block gene expression and probe chromosomal DNA. Here we describe a protocol for designing antigene agents and introducing them into cells. This protocol can also be used to silence expression with PNAs or siRNAs that target mRNA. From preparation of oligomers to analysis of data, experiments with agPNAs and agRNAs require similar to 14 d and 9 d, respectively.
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