Journal
JOURNAL OF BIOMECHANICS
Volume 39, Issue 1, Pages 78-87Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2004.11.006
Keywords
cell mechanics; cartilage; biphasic; viscoelastic; nucleus; ostcoarthritis; arthritis
Categories
Funding
- NIAMS NIH HHS [AR50245, AR48182] Funding Source: Medline
- NIA NIH HHS [AG15768] Funding Source: Medline
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [P01AR050245, R01AR048182] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG015768] Funding Source: NIH RePORTER
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Chondrocytes in articular cartilage are regularly subjected to compression and recovery due to dynamic loading of the joint. Previous studies have investigated the elastic and viscoelastic properties of chondrocytes using micropipette aspiration techniques, but in order to calculate cell properties, these studies have generally assumed that cells are incompressible with a Poisson's ratio of 0.5. The goal of this study was to measure the Poisson's ratio and recovery properties of the chondrocyte by combining theoretical modeling with experimental measures of complete cellular aspiration and release from a micropipette. Chondrocytes isolated from non-osteoarthritic and osteoarthritic cartilage were fully aspirated into a micropipette and allowed to reach mechanical equilibrium. Cells were then extruded from the micropipette and cell volume and morphology were measured throughout the experiment. This experimental procedure was simulated with finite element analysis, modeling the chondrocyte as either a compressible two-mode viscoelastic solid, or as a biphasic viscoelastic material. By fitting the experimental data to the theoretically predicted cell response, the Poisson's ratio and the viscoelastic recovery properties of the cell were determined. The Poisson's ratio of chondrocytes was found to be 0.38 for non-osteoarthritic cartilage and 0.36 for osteoarthritic chondrocytes (no significant difference). Osteoarthritic chondrocytes showed an increased recovery time following full aspiration. In contrast to previous assumptions, these findings suggest that chondrocytes are compressible, consistent with previous studies showing cell volume changes with compression of the extracellular matrix. (c) 2004 Elsevier Ltd. All rights reserved.
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