Transforming growth factor-beta 2 suppresses collagen cleavage in cultured human osteoarthritic cartilage, reduces expression of genes associated with chondrocyte hypertrophy and degradation, and increases prostaglandin E-2 production

Articular cartilage degeneration in ostcoarthritis (OA) involves type H collagen degradation and chondrocyte differentiation (hypertrophy). Because these changes resemble growth plate remodeling, we hypothesized that collagen degradation may be inhibitable by growth factors known to suppress growth plate hypertrophy, namely transforming growth factor (TGF)-beta 2, fibroblast growth factor (FGF)-2, and insulin. Full-depth explants of human OA knee articular cartilage from arthroplasty were cultured with TGF-beta 2, FGF-2, and insulin in combination (growth factors) or individually. In cultured explants from five OA patients, collagenase-mediated type 11 collagen cleavage was significantly down-regulated by combined growth factors as measured by enzyme-linked inummosorbent assay. individually, FGF-2 and insulin failed to inhibit collagen cleavage in some OA explants whereas TGF-beta 2 reduced collagen cleavage in these 5 explants and in 19 additional explants. Moreover, TGF-beta 2 effectively suppressed cleavage at low concentrations. Together or individually these growth factors did not inhibit glycosaminoglycan (primarily aggrecan) degradation while TGF-beta 2 occasionally did. Semiquantitative reverse transcriptase-polymerase chain reaction of articular cartilage from six OA patients revealed that TGF-beta 2 suppressed expression of matrix metalloproteinase-13 and matrix metalloproteinase-9, early (PTHrP) and late (COL10A1) differentiation-related genes, and proinflammatory cytokines (interleukin-1,6, tumor necrosis factor-alpha). In contrast, TGF-beta 2 up-regulated PGES-1 expression and prostaglandin E-2 release. These observations show that TGF-beta 2 can suppress collagen resorption and chondrocyte differentiation in OA cartilage and that this may be mediated by prostaglandin E-2. Therefore TGF-beta 2 could provide therapeutic control of type II collagen degeneration in OA.