Multi-walled carbon nanotubes-based glucose biosensor prepared by a layer-by-layer technique

The loading of multi-walled carbon nanotubes (MWNTs) and glucose oxidase (GOx) in the alternate layers of a glucose biosensor was first optimized based on a layer-by-layer construction on the surface of a graphite disk electrode. With the increasing of MWNTs/GOx layers, the response current to glucose was changed regularly and the response current reached a maximum value when the number of MWNTs/GOx layers was 6. Owing to a good electrical conductivity, strong adsorption and excellent bioconsistency of MWNTs, the (MWNTs/GOx)(6) films-coated glucose biosensor had an excellent electrochemical properties. The response current of the (MWNTs/GOs)(6) films-coated biosensor to 3 x 10(-2) M glucose was 1.63 mu A while the response time was only 6.7 s. The linear range and the lowest detectable concentration of this biosensor was 5 x 10(-4) similar to 1.5 x 10(-2) M and 0.9 x 10(-4) M, respectively. (c) 2005 Elsevier B.V. All rights reserved.