Journal
BIOTECHNIQUES
Volume 40, Issue 1, Pages 85-90Publisher
EATON PUBLISHING CO
DOI: 10.2144/000112071
Keywords
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Funding
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG002644] Funding Source: NIH RePORTER
- NHGRI NIH HHS [1R01 HG002644-01A1] Funding Source: Medline
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Here we describe the development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system. A 100-chamber polydimethylsiloxane (PDMS) chip performs up to 5 tests for each of 10 samples. In this particular study system, the specificity of detection was demonstrated, and calibration curves were produced for C-reactive protein (CRP), prostate-specific antigen (PSA), ferritin, and vascular endothelial growth factor (VEGF). The measurements show sensitivity at and below clinically normal levels (with a signal-to-noise ratio >8 at as low as 10 pM antigen concentration). The chip uses 100 nL per sample for all tests. The developed system is an important step toward derivative immunoassay applications in scientific research and point-of-care testing in medicine.
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