Journal
STEM CELLS
Volume 24, Issue 1, Pages 168-176Publisher
WILEY
DOI: 10.1634/stemcells.2005-0292
Keywords
human embryonic stem cells; reprogramming; myeloid precursors; cell fusion; hybrid cells
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Funding
- NCRR NIH HHS [P51 RR000167] Funding Source: Medline
- NIGMS NIH HHS [P20 GM069981] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P51RR000167] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM069981] Funding Source: NIH RePORTER
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Here, we examine the ability of undifferentiated human embryonic stem cells (hESCs) to reprogram the nuclei of hESC-derived myeloid precursors following cell-cell fusion. Using an OP9 coculture system, we produced CD45(+)CD33(+) myeloperoxidase(+) myeloid precursors from an Oct4-enhanced green fluorescent protein (EGFP) knock-in hESC line and demonstrated that Oct4-EGFP expression was extinguished in these precursors. Upon fusion with undifferentiated hESCs, EGFP expression from the endogenous Oct4 promoter/regulatory region was re-established, ESC-specific surface antigens and marker genes were expressed, and myeloid precursor-specific antigens were no longer detectable. When the hybrid cells were formed into embryoid bodies, upregulation of genes characteristic of the three germ layers and extraembryonic tissues occurred, indicating that the hybrid cells had the potential to differentiate into multiple lineages. Interestingly, the hybrid cells were capable of redifferentiating into myeloid precursors with efficiency comparable with that of diploid hESCs despite their near-tetraploid chromosome complement. These results indicate that hESCs are capable of reprogramming nuclei from differentiated cells and that hESC hybrid cells provide a new model system for studying the mechanisms of nuclear reprogramming.
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