4.4 Article

Purification and characterization of a cold-active iron superoxide dismutase from a psychrophilic bacterium, Marinomonas sp NJ522

Journal

BIOTECHNOLOGY LETTERS
Volume 28, Issue 2, Pages 85-88

Publisher

SPRINGER
DOI: 10.1007/s10529-005-4951-3

Keywords

Antarctic; cold-active; Marinomonas; psychrophilic bacteria; superoxide dismutase

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Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degrees C was nearly 35% of the maximum activity.

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