Journal
CLINICAL BIOCHEMISTRY
Volume 40, Issue 5-6, Pages 411-413Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2006.12.001
Keywords
biopterin; tetrahydrobiopterin; plasma; HPLC; humans
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Objective: To develop a reliable method for the determination of tetrahydrobiopterin and total biopterin in plasma. Methods: Modifications include blood collection and handling procedures. Results: Collection of blood in the presence of 0.1% (w/v) dithiothreitol and waiting for 2-3 h before centrifugation are optimal for adequate quantitation of both pterins. Conclusions: For a reliable determination of tetrahydrobiopterin and total biopterin in plasma, blood tubes must contain a dimercapto-antioxidant and time between blood collection and centrifugation is critical. (c) 2007 Published by The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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