Amperometric biosensors based on platinum nanowires

Platinum nanowires (PtNW) were prepared by an electrodeposition strategy using nanopore alumina template. The nanowires prepared were dispersed in chitosan (CHIT) solution and stably immobilized onto the surface of glassy carbon electrode (GCE). The electrochemical behavior of PtNW-modified electrode and its application to the electrocatalytic reduction of hydrogen peroxide (H2O2) are investigated. The modified electrode allows low potential detection of hydrogen peroxide with high sensitivity and fast response time. As an application example, the glucose oxidase was immobilized onto the surface of PtNW-modified electrode through cross-linking by glutaric dialdehyde. The detection of glucose was performed in phosphate buffer at -0.2 V. The resulting glucose biosensor exhibited a short response time (< 8 s), with a linear range of 10(-5) x 10(-6) M and detection limit of 5 x 10(-6) M.