Journal
EXPERIMENTAL EYE RESEARCH
Volume 84, Issue 1, Pages 75-81Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2006.08.018
Keywords
retina; choroid; adrenergic receptors; aging
Categories
Funding
- NIA NIH HHS [R15AG027827A] Funding Source: Medline
Ask authors/readers for more resources
While age-related night vision loss and age-related macular degeneration are well characterized, less is known about the normal aging process in the retina and choroid. The purpose of this study was to ascertain whether dopamine beta-hydroxylase (DBH), beta(1)- and beta(2)-adrenergic receptor gene and protein expression are altered in the retina and choroid with age. The retina and choroid were dissected from F344 x BNF1 hybrid rats aged 8, 22, and 32 months. Real-time PCR and Western blot analysis were conducted to determine steady-state mRNA and protein expression. Immunohistochemistry (IHC) was conducted to localize DBH protein expression in the retina. DBH protein expression was substantially decreased with age in the retina, particularly in the outer nuclear layer, with no changes in DBH expression noted in the choroid. There was a significant increase in beta(1)-adrenergic receptor protein expression in retinal samples at 22 months, while beta(2)-adrenergic receptor protein expression was not affected by age. Decreased expression of DBH with age in the retina could lead to reduced production of norepinephrine, potentially resulting in an increase of beta(1)-adrenergic receptor expression due to denervation supersensitivity. Gene expression for DBH, beta(1)- and beta(2)-adrenergic receptors were observed to peak at 22 months and return to baseline levels by 32 months of age in the choroid. Our findings suggest that the retina may be more sensitive to age-related loss of sympathetic neurotransmission than the choroid, which may partially explain normal age-related vision loss in the elderly. (c) 2006 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available