Journal
ANALYTICAL LETTERS
Volume 40, Issue 6, Pages 1063-1070Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710701297133
Keywords
fluorescence polarization; DNA hybridization; mismatch detection
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The potential of fluorescent polarization analysis as a method for detection of mismatch DNA hybridization was investigated. The dependency of DNA hybridization rate on salt concentration was surveyed. In greater than 0.1 M NaCl, the hybridization of probe and target DNA proceeds rapidly and the reaction is complete within 3 min. Furthermore, the hybridization of probe DNA and one mismatch target DNAs was investigated. It was successfully shown that even one mismatch could be detected using fluorescence polarization analysis if the mismatch position was on the base that pairs with the probe DNA at the 5 ' terminus where fluorescein isothiocyanate (FITC) is attached.
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