Journal
JOURNAL OF RAMAN SPECTROSCOPY
Volume 38, Issue 4, Pages 369-372Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/jrs.1721
Keywords
Raman spectroscopy; fluorescence staining; microorganisms
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The successful combination of Raman spectroscopy and fluorescence labelling of single bacterial cells is presented. The results show that the selected dyes (DAPI, SYTO 9, CFDA, SYTOX blue and PI) in most cases do not influence the Raman spectrum of a labelled bacterium significantly compared to an unstained bacterium spectrum. The labelling of bacteria offers the possibility of live/dead or biotic/abiotic differentiation prior to a Raman identification step. Copyright (c) 2007 John Wiley & Sons, Ltd.
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