Structural and mechanistic insights into hepatitis C viral translation initiation

Hepatitis C virus uses an internal ribosome entry site (IRES) to control viral protein synthesis by directly recruiting ribosomes to the translation-start site in the viral mRNA. Structural insights coupled with biochemical studies have revealed that the IRES substitutes for the activities of translation-initiation factors by binding and inducing conformational changes in the 40S ribosomal subunit. Direct interactions of the IRES with initiation factor elF3 are also crucial for efficient translation initiation, providing clues to the role of elF3 in protein synthesis.