Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 157, Issue 1, Pages 117-125Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2006.05.004
Keywords
image processing; electron microscopy; protein structure; FREALIGN
Funding
- NIGMS NIH HHS [1 P01 GM-62580] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P01GM062580] Funding Source: NIH RePORTER
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The refinement of three-dimensional reconstructions and correction for the contrast transfer function of the microscope are important steps in the determination of macromolecular structures by single particle electron microscopy. The algorithms implemented in the computer program FREALIGN are optimized to perform these tasks efficiently. A general overview and details on how to use FREALIGN are provided. The program is free and available for download on the author's web page. (c) 2006 Elsevier Inc. All rights reserved.
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