Immunostimulatory principles from Chlorella pyrenoidosa - Part 1: Isolation and biological assessment in vitro

Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin (TM)), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin (TM) is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin (TM) is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose. (c) 2005 Elsevier GmbH. All rights reserved.