Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 157, Issue 1, Pages 201-210Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2006.08.003
Keywords
resolution measurement; electron microscopy; protein structure; single particle; RMEASURE
Funding
- NIGMS NIH HHS [P01 GM-62580] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P01GM062580] Funding Source: NIH RePORTER
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A computational method is described that allows the measurement of the signal-to-noise ratio and resolution of a three-dimensional structure obtained by single particle electron microscopy and reconstruction. The method does not rely on the availability of the original image data or the calculation of several structures from different parts of the data that are needed for the commonly used Fourier Shell Correlation criterion. Instead, the correlation between neighboring Fourier pixels is calculated and used to distinguish signal from noise. The new method has been conveniently implemented in a computer program called RMEASURE and is available to the microscopy community. (c) 2006 Elsevier Inc. All rights reserved.
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