Journal
CHEMISTRY & BIOLOGY
Volume 14, Issue 1, Pages 107-115Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2006.11.015
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Funding
- NCI NIH HHS [N01-CO-27173] Funding Source: Medline
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Dendrimer-based anticancer nanotherapeutics containing similar to 5 folate molecules have shown in vitro and in vivo efficacy in cancer cell targeting. Multivalent interactions have been inferred from observed targeting efficacy, but have not been experimentally proven. This study provides quantitative and systematic evidence for multivalent interactions between these nanodevices and folate-binding protein (FBP). A series of the nanodevices; were synthesized by conjugation with different amounts of folate. Dissociation constants (K-D) between the nanodevices and FBP measured by SPR are dramatically enhanced through multivalency (similar to 2,500- to 170,000-fold). Qualitative evidence is also provided for a multivalent targeting effect to KB cells using flow cytometry. These data support the hypothesis that multivalent enhancement of K-D, not an enhanced rate of endocytosis, is the key factor resulting in the improved biological targeting by these drug delivery platforms.
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