Journal
EXPERT REVIEW OF PROTEOMICS
Volume 4, Issue 2, Pages 187-198Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1586/14789450.4.2.187
Keywords
bacteria; chromatography; data analysis; metabolome; metabolomic; sample preparation; triple quadrupole mass spectrometry
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Funding
- NIGMS NIH HHS [P50 GM071508] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P50GM071508] Funding Source: NIH RePORTER
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Escherichia coli is among the simplest and best-understood free-living organisms. It has served as a valuable model for numerous biological processes, including cellular metabolism. Just as E coli stood at the front of the genomic revolution, it is playing a leading role in the development of cellular metabolomics: the study of the complete metabolic contents of cells, including their dynamic concentration changes and fluxes. This review briefly describes the essentials of cellular metabolomics; and its fundamental differentiation from biomarker metabolomics and lipidomics. Key technologies for metabolite quantitation from E coli are described, with a focus on those involving mass spectrometry. In particular emphasis is given to the cell handling and sample preparation steps required for collecting data of high biological reliability, such as fast metabolome quenching. Future challenges, both in terms of data collection and application of the data to obtain a comprehensive understanding of metabolic dynamics, are discussed.
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