Journal
CANCER LETTERS
Volume 348, Issue 1-2, Pages 119-125Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2014.03.014
Keywords
Urinary bladder cancer; Bacillus Calmette-Guerin; Nitric oxide; Nitric oxide synthase; Cytokines
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Funding
- Swedish society of Medicine
- Cancer Society in Stockholm
- Stockholm County Council
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The anti-tumour mechanisms following Bacillus Calmette-Guerin (BCG) treatment of bladder-cancer remain largely unknown. Previous studies have shown involvement of nitric-oxide (NO) formation in the BCG-mediated effect. We analyzed the effects of macrophage secreted factors (MSFs) from BCG-stimulated RAW264.7 cells on the bladder-cancer cell line MBT2. Direct treatment with BCG did not induce NO in MBT2-cells whereas supernatant from BCG-stimulated macrophages increased NOS2 mRNA and protein expression, NO concentrations and cell-death. Blocking NO-synthesis with the NOS-inhibitor L-NAME did not affect levels of cell-death suggesting cytotoxic pathways involving other signalling molecules than NO. Several such candidate genes were identified in a microarray. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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