Journal
CANCER LETTERS
Volume 328, Issue 1, Pages 126-134Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2012.08.032
Keywords
TMEM166; Adenovirus vector; Autophagy; Apoptosis; Anti-tumor activity
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Funding
- National Key Project for Basic Research of China (973) [2011CB910103]
- National Natural Science Foundation of China [30771057]
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TMEM166 is a novel programmed cell death-related molecule. In this report, we constructed a recombinant adenovirus 5-TMEM166 vector (Ad5-TMEM166) and evaluated its expression and anti-tumor activities in vitro and in vivo. Cell viability analysis revealed that the adenovirus-mediated increase of TMEM166 inhibited tumor cell growth in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy (via inhibition of mTOR and activation of p70S6K) and apoptosis (via caspase-3 activation), both of which contributed to cell death and suppression of tumorigenicity. Our data indicated that Ad5-TMEM166 may be a novel gene therapy candidate for cancer. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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