Journal
CANCER LETTERS
Volume 317, Issue 2, Pages 184-191Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2011.11.024
Keywords
MiR-100; Plk1; Lung adenocarcinoma; Docetaxel; Chemoresistance
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Funding
- National Natural Science Foundation of China [81071806, 81172106]
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MicroRNAs (miRNAs) expression correlates with biological characteristics of both normal cells and cancer cells, but their roles in cancer chemoresistance remain unclear. By microarray analysis, miR-100 was found significantly down-regulated in docetaxel-resistant SPC-A1/DTX cells compared with parental SPC-A1 cells. Ectopic miR-100 expression resensitized SPC-A1/DTX cells to docetaxel by suppression of cell proliferation and induction of cell arrest in G(2)/M phase and apoptosis. Knock-down of Plk1, which was a direct target of miR-100, yielded similar effects as that of ectopic miR-100 expression. The inverse correlation between miR-100 and Plk1 expression was also detected in nude mice SPC-A1/DTX tumor xenografts and clinical lung adenocarcinoma tissues and was proved to be related with the in vivo response to docetaxel. Thus, our results suggested that down-regulation of miR-100 could lead to Plk1 over-expression and eventually to docetaxel chemoresistance of human lung adenocarcinoma. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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