Journal
CANCER LETTERS
Volume 310, Issue 1, Pages 1-8Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2011.04.009
Keywords
Epithelial-mesenchymal transition; EGF; Resveratrol; MCF-7
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Funding
- ARTI strategic project of Apulia Region
- Angela Serra association, Parabita (Lecce, Italy)
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Carcinoma progression is associated with the loss of epithelial features, and the acquisition of a mesenchymal phenotype by tumour cells. Herein we show that exposure of MCF-7 cells to epidermal growth factor (EGF) resulted in morphological alterations characteristic of epithelial-to-mesenchymal transition (EMT). EGF treatment resulted in increased motility along with an up-regulation of transcription factors Slug, Zeb1, Zeb2, and mesenchymal markers Vimentin and N-cadherin. Treatment of MCF-7 cells with a combined stimulation of EGF and resveratrol, a naturally occurring stilbene with antitumor properties, failed to alter cell morphology, motility and overexpression of EMT markers induced by EGF. Using specific chemical inhibitors, we demonstrated that EGF-induced EMT is mediated by extracellular signal-regulated kinase 1/2 (ERK 1/2) signalling pathway and that resveratrol is able to repress EGF-induced ERK activation. In summary, these data provide new evidence of the inhibitory effect of resveratrol on EGF-induced EMT cell transformation. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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