Journal
CANCER LETTERS
Volume 300, Issue 1, Pages 96-104Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2010.09.015
Keywords
Fisetin; Securin; p53; Human colon cancer cells
Categories
Funding
- National Science Council Taiwan [NSC 99-2314-B-320-004-MY3]
Ask authors/readers for more resources
Securin is highly-expressed in various tumors including those of the colon In this study the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay Annexin V-FITC/PI double staining Ser15-phosphorylation of p53 and cleavages of procaspase-3 and PARP These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA but attenuated when wild-type or non-degradable securin was reconstituted Moreover fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells Knockdown of securin in HCT116 p53-null cells potentiated fisetin-induced cytotoxicity by induction of apoptosis Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis (c) 2010 Elsevier Ireland Ltd All rights reserved
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available