Journal
CANCER LETTERS
Volume 288, Issue 1, Pages 99-106Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2009.06.027
Keywords
Micrometastasis; Circulating tumor cells; Breast cancer; Stem cells; Triple-marker immunofluorescence
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Funding
- Cretan Association for Biomedical Research (CABR)
- KESY
- Greek Ministry of Health [KA2340, KA2789]
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The CD44(+)/CD24(-/low) and ALDH1(+) cell phenotypes are associated with sternness and enhanced tumorigenic potential in breast cancer. We assessed the expression of CD44, CD24 and ALDH1 on tumor cells circulating in the peripheral blood (CTCs) of patients with metastatic breast cancer using triple-marker immurrofluorescence microscopy. Among a total of 1439 CTCs identified in 20 (66.7%) out of 30 patients, 35.2% had the stem-like/tumorigenic phenotype CD44(+)/CD24(-/low), whereas 17.7% of the CTCs analyzed in seven patients, were ALDH1(high)/CD24(-/low). In conclusion, we report the existence of a subpopulation of CTCs with putative stem cell progenitor phenotypes in patients with metastatic breast cancer. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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