Pharmacological evaluation of Propolis solutions for endodontic use

This study evaluated the anti-inflammatory and exudative activities of propolis solutions and their antimicrobial activity. The solutions were prepared and diluted in alcohol solution (PPE1, PPE2, PPE3, PPF18, and PPF19). Ear edema was previously induced in mice by the application of croton oil, and the irritative effect of the solutions was determined through the exudation test of Evans blue in rats. Antimicrobial activity by using a macrodilution method was determined. Eight aerobic bacteria, seven anaerobic bacteria, and two yeasts were tested. The PPE1, PPE2, and PPF18 solutions presented excellent anti-inflammatory activities. PPE1 solution showed the best antimicrobial effect. PPF18 showed an inhibitory effect for the majority of the aerobic bacteria in the dilution 1:8, inhibiting the growth of Klebsiella pneumoniae and the yeasts in the dilution 1/16 and of Pseudomones aeruginosa in the dilution 1/32. PPF19 was effective for inhibiting the growth of the aerobic bacteria and yeasts in the dilution 1/2. Our results suggest the possible application of PPE1, PPF18, and PPF19 solutions in endodontics.