Journal
CANCER LETTERS
Volume 281, Issue 1, Pages 64-70Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2009.02.021
Keywords
Lung cancer; Peptide; Phage display; Biomarker; NCI-H1299; Phage M13
Categories
Funding
- National Natural Science Foundation of China [30572177]
- National Education Ministry Key Research Project of China [208105]
- Initiation Research Project of Guangdong Pharmaceutical University, PR China [2006]
- Middle-youth key Teacher Project of Guangdong Pharmaceutical University [2008]
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In this study, a NCI-H1299 (Non-Small Cell Lung Cancer, NSCLC) and a normal lung cell line (Small Airway Epithelial Cells, SAEC) were used for the subtractive screening in vitro with a phage display-12 peptide library. After three rounds of panning, there was an obvious enrichment for the phages specifically binding to the NCI-H1299 cells, and the output/input ratio of phages increased about 875-fold (from 0.4 x 10(4) to 3.5 x 10(6)). A group of peptides being capable of binding specifically to the NCI-H1299 cells were obtained, and the affinity of these peptides to bind to the targeted cells and tissues was studied. Through a cell-based ELISA, immunocytochemical staining, immunohistochemical staining, and immunofluorescence, a M13 phage isolated and identified from the above screenings, and a synthetic peptide ZS-1 (sequence EHMALTYPFRPP) corresponded to the sequence of the surface protein of the M13 phage were demonstrated to be capable of binding to the tumor cell surfaces of NCI-H1299 and A549 cell lines and biopsy specimens, but not to normal lungs tissue samples, other different cancer cells, or nontumor surrounding lung tissues. In conclusion, the peptide ZS-1 may be a potential candidate of biomarker ligands used for targeted drug delivery in therapy of lung cancer. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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