Journal
RESEARCH IN MICROBIOLOGY
Volume 158, Issue 3, Pages 187-194Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2006.12.008
Keywords
fluorescence; green fluorescent protein (GFP); fluorescence resonant energy transfer (FRET); fluorescence recovery after photobleaching (FRAP); bacteria; microscopy; single cell; time lapse
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Fluorescence-imaging techniques are now being applied to single bacterial cells for the determination of in vivo kinase and transcription activity, protein-protein interactions, protein localization and protein mobility. Time-lapse imaging is being used at a population level to study heterogeneities, colony morphologies and the genetic networks underlying these phenomena. We will discuss here the possibilities, advantages and limitations of such techniques, focusing on the use of green fluorescent protein and its spectral variants. (c) 2007 Elsevier Masson SAS. All rights reserved.
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