Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 11, Issue 2, Pages 203-213Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2007.02.025
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The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the,old yellow enzyme' family at the expense of NAD(P)H yields the corresponding non-racemic alkanes going in hand with the creation of up to two chiral carbon centres. To avoid external cofactor recycling, this intriguing biotransformation was hitherto performed using whole microbial cells, which frequently showed insufficient stereoselectivities and/or undesired side reactions because of the action of competing enzymatic activities. Co-expression of enoate reductases with the corresponding redox enzymes for NAD(P)H recycling in a suitable host enables to overcome these drawbacks to furnish highly stereoselective and 'clean' C=C bioreductions on a preparative scale that are difficult to perform by conventional means.
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