Journal
CANCER IMMUNOLOGY IMMUNOTHERAPY
Volume 59, Issue 9, Pages 1425-1433Publisher
SPRINGER
DOI: 10.1007/s00262-010-0824-2
Keywords
MHC multimers; Combinatorial encoding; T cell detection; Epitope mapping; MHC tetramers; Quantum dots
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The hallmark of adaptive immunity is its ability to recognise a wide range of antigens and technologies that capture this diversity are therefore of substantial interest. New methods have recently been developed that allow the parallel analysis of T cell reactivity against vast numbers of different epitopes in limited biological material. These technologies are based on the joint binding of differentially labelled MHC multimers on the T cell surface, thereby providing each antigen-specific T cell population with a unique multicolour code. This strategy of 'combinatorial encoding' enables detection of many (at least 25) different T cell populations per sample and should be of broad value for both T cell epitope identification and immunomonitoring.
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