Susceptibility to inhalation toxicity of acetaldehyde in Aldh2 knockout mice

In this study, we evaluated the inhalation toxicity of acetaldehyde in Aldh2 KO ( Aldh-/-) mice, using pathological method. Male C57BL/6 ( Aldh+/+) mice and Aldh -/- mice were exposed to atmospheres containing acetaldehyde at levels of 0, 125, and 500 ppm for 24 h/day during 14 days. Although the average blood acetaldehyde concentration of Aldh -/- mice was higher than that of Aldh2+/+ mice in the acetaldehyde exposure group, observable effects by the acetaldehyde exposure on the lung and liver were not different between wild type and ALDH2 null mice. In Aldh2-/- mice, the levels of 1) erosion of respiratory epithelium and the subepithelial hemorrhage in nose, 2) hemorrhage in nasal cavity, 3) degeneration of respiratory epithelium in larynx, pharynx and trachea, and 4) degeneration of dorsal skin were higher compared with Aldh2+/+ mice, indicating that Aldh2-/- mice are more acetaldehyde-sensitive than Aldh2+/+ mice. This is the first example for studying pathological effects of Aldh2 deficiency using Aldh-/- mice exposed to a low level of acetaldehyde.