Journal
INTERVIROLOGY
Volume 50, Issue 3, Pages 224-228Publisher
KARGER
DOI: 10.1159/000100565
Keywords
HIV Tat; Bcl-2; monocytes; apoptosis; TRAIL
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI038491, R01AI046244] Funding Source: NIH RePORTER
- NIAID NIH HHS [R01 AI038491-05, R01 AI046244-03, R01 AI046244-04, R01 AI046244-01A1, R01 AI038491-04, R01 AI046244-02] Funding Source: Medline
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Objective: To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha- related apoptosis-induced ligand (TRAIL). Methods: Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexin V and 7-AAD staining were used to detect apoptosis of monocytes induced by TRAIL. Results: HIV Tat protein increased Bcl-2 expression in human monocytes in a dose-dependent manner. Annexin V staining showed that 51.54% of monocytes underwent apoptosis after being treated with 100 ng/ml recombinant TRAIL. When monocytes were prestimulated with HIV Tat, only 15.46% of monocytes underwent apoptosis. This effect can be inhibited by polyclonal anti-Tat serum. 7-AAD staining showed similar results. Conclusion: HIV Tat protein increases Bcl-2 expression in monocytes which inhibited apoptosis induced by TRAIL. HIV Tat protein may play an important role in the mechanisms of HIV-persistent infection in monocytes.
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