A rapid determination method for anthocyanin profiling in grape genetic resources

A rapid determination method has been developed to elucidate the anthocyanin profiles in grape genetic resources. It consists of crude extraction of skin anthocyanins with 50% aqueous acetic acid and analysis of reversed-phase HPLC with a visible spectrum detector. A total of 21 anthocyanins could be rapidly identified without the need for multi-step extraction and equipment such as diode array spectroscopy and/or mass spectrometry. Analysis of 17 colored cultivars exhibited diversified anthocyanin profiles in the levels of hydroxylation, methylation, glycosidation, and acylation of aglycones, suggesting that significant inter- and intraspecific variations exist in grape genetic resources. The anthocyanin profiles for a grape cultivar from the same location appeared to be quite similar between vines or years.