Journal
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
Volume 29, Issue 3-4, Pages 563-568Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/08923970701691033
Keywords
gold nanoparticles; clenbuterol; antibodies; antigen carrier; immunodot assay
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The effectiveness of an in vivo synthesis of antibodies to clenbuterol with the use of gold nanoparticles as a carrier was evaluated. For comparison, conjugates of clenbuterol with bovine serum albumin were used in immunization. The serum titer was determined by an ELISA. The antibodies were tested by an immunodot assay with immunogold markers. With both techniques we obtained specific and relatively high-titer antibodies to clenbuterol. It was found that the antibodies obtained with the use of gold nanoparticles were not inferior in titer to those obtained to the conjugates of clenbuterol with the protein but surpassed them in specificity.
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