Journal
CANCER CYTOPATHOLOGY
Volume 122, Issue 10, Pages 779-785Publisher
WILEY
DOI: 10.1002/cncy.21458
Keywords
melanoma; pan-melanoma; Sry-related HMG-BOX gene 10 (SOX10); microphthalmia transcription factor (MITF); fine-needle aspiration (FNA); cytology; smear
Funding
- University of Texas MD Anderson Cancer Center
Ask authors/readers for more resources
BACKGROUNDAccurate fine-needle aspiration (FNA) diagnosis of metastatic melanoma is of therapeutic and prognostic significance and often requires ancillary studies. To the authors' knowledge, the reliability of immunostaining using a pan-melanoma cocktail, Sry-related HMG-BOX gene 10 (SOX10), and microphthalmia transcription factor (MITF) in confirming a diagnosis of melanoma on FNA smears has not been studied to date. METHODSThis retrospective study included 50 FNA cases with a definitive diagnosis of melanoma. Twenty-nine cases were epithelioid type (group 1), and 21 cases were predominantly spindle cell type with or without an epithelioid component (group 2). Each case was immunostained using pan-melanoma cocktail, SOX10, and MITF. Staining intensity and the percentage of positive cells were recorded. RESULTSThe pan-melanoma cocktail was positive in 43 cases (86%), SOX10 was positive in 50 cases (100%), and MITF in 45 cases (90%). SOX10 and MITF demonstrated nuclear staining with stronger and more diffuse staining with less or no background staining compared with pan-melanoma cocktail, which displayed cytoplasmic staining. For pan-melanoma cocktail and SOX10, the detection rates were identical in groups 1 and 2 (86% with pan-melanoma cocktail and 100% with SOX10). For MITF, the detection rate was higher in group 1 compared with Group 2 (93% vs 86%). CONCLUSIONSIn the current study, SOX10 was found to have the highest overall detection rate, followed by MITF and pan-melanoma cocktail. The pan-melanoma cocktail and SOX10 performed equally well for groups 1 and 2, and MITF had a higher detection rate in group 1. Overall, SOX10 and MITF appeared to be superior to the pan-melanoma cocktail and SOX10 seemed better than MITF in confirming a diagnosis of melanoma on FNA smears. Cancer (Cancer Cytopathol) 2014;122:779-785. (c) 2014 American Cancer Society. In the current study, SOX10 was found to have the highest detection rate for melanoma on cytology smears, followed by microphthalmia transcription factor (MITF) and pan-melanoma cocktail. The nuclear staining markers, SOX10 and MITF, appear to be more readily interpreted than pan-melanoma cocktail.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available