Journal
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
Volume 28, Issue 3, Pages 279-288Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15321810701454870
Keywords
Leptospira; recombinant antigens; antibody affinity; epitope mapping; ELISA; western blotting
Funding
- FIC NIH HHS [TW00919] Funding Source: Medline
- NIAID NIH HHS [AI034431] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI034431, R21AI034431, R29AI034431] Funding Source: NIH RePORTER
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LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies ( MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG2b isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5 x 10(7) M-1 and 6 x 10(6) M-1. These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection.
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