Journal
ANNUAL REVIEW OF BIOCHEMISTRY
Volume 76, Issue -, Pages 223-241Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.biochem.76.061505.175355
Keywords
copper switch; methanobactin; methanotroph; particulate methane; monooxygenase
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Funding
- NIGMS NIH HHS [GM070473] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM070473] Funding Source: NIH RePORTER
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Methanotrophic bacteria oxidize methane to methanol in the first step of their metabolic pathway. Two forms of methane monooxygenase (MMO) enzymes catalyze this reaction: soluble MMO (sMMO) and membrane-bound or particulate MMO (pMMO). pMMO is expressed when copper is available, and its active site is believed to contain copper. Whereas sMMO is well characterized, most aspects of pMMO biochemistry remain unknown and somewhat controversial. This review emphasizes advances in the past two to three years related to pMMO and to copper uptake and copper-dependent regulation in methanotrophs. The pMMO metal centers have been characterized spectroscopically, and the first pMMO crystal structure has been determined. Significant effort has been devoted to improving in vitro pMMO activity. Proteins involved in sMMO regulation and additional copper-regulated proteins have been identified, and the Methylococcus capsulatus (Bath) genome his been sequenced. Finally, methanobactin (nib), a small copper chelator proposed to facilitate copper uptake, has been characterized.
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