Journal
INFLUENZA AND OTHER RESPIRATORY VIRUSES
Volume 1, Issue 1, Pages 19-25Publisher
WILEY
DOI: 10.1111/j.1750-2659.2006.00005.x
Keywords
Carrier molecule; influenza vaccine; launch vector; lichenase; plant viral expression; subunit vaccine
Categories
Ask authors/readers for more resources
Historically, most vaccines have been based on killed or live-attenuated infectious agents. Although very successful at immunizing populations against disease, both approaches raise safety concerns and often have limited production capacity. This has resulted in increased emphasis on the development of subunit vaccines. Several recombinant systems have been considered for subunit vaccine manufacture, including plants, which offer advantages both in cost and in scale of production. We have developed a plant expression system utilizing a 'launch vector', which combines the advantageous features of standard agrobacterial binary plasmids and plant viral vectors, to achieve high-level target antigen expression in plants. As an additional feature, to aid in target expression, stability and purification, we have engineered a thermostable carrier molecule to which antigens are fused. We have applied this launch vector/carrier system to engineer and express target antigens from various pathogens, including, influenza A/Vietnam/04 ( H5N1) virus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available