Journal
ANNUAL REVIEW OF PHYSICAL CHEMISTRY
Volume 58, Issue -, Pages 489-510Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.physchem.58.032806.104427
Keywords
chromatography; peak tailing; isolated silanols; topography
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Single-molecule spectroscopy has emerged as a valuable tool in probing kinetics and dynamic equilibria in adsorption because advances in instrumentation and technology have enabled researchers to obtain high signal-to-noise ratios for common dyes at room temperature. Single-molecule spectroscopy was applied to the study of an important problem in chromatography: peak broadening and asymmetry in the chromatograms of pharmaceuticals, peptides, and proteins. Using DiI, a cationic dye that exhibits the same problematic chromatographic behavior, investigators showed that the adsorption sites that cause chromatographic problems are located at defects on the silica crystal surface.
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