Journal
CANCER
Volume 120, Issue 3, Pages 363-372Publisher
WILEY
DOI: 10.1002/cncr.28418
Keywords
nasopharyngeal carcinoma; Epstein-Barr virus; microRNA; epithelial-mesenchymal transition; metastasis
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Funding
- National Natural Science Foundation of China [30772469, 81172053, 30801381]
- Natural Science Foundation of Guangdong Province, China [10151051501000068]
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BACKGROUNDThe Epstein-Barr virus (EBV)-encoded EB nuclear antigen 1 (EBNA1) protein is required for maintenance and transmission of the viral episome in EBV-infected cells. The objective of this study was to investigate the role of EBNA1 protein in nasopharyngeal carcinoma (NPC). METHODSTissue samples from 48 patients with NPC and 12 patients with chronic nasopharyngitis were subjected to immunohistochemical analysis of EBNA1 expression. EBNA1 combinational DNA was used to overexpress EBNA1 protein in NPC cell lines to assess tumor cell epithelial-mesenchymal transition (EMT), colony formation, migration and invasion, and gene expression. RESULTSEBNA1 protein was highly expressed in NPC tissue specimens, and its expression was associated with NPC lymph node metastasis. EBNA1 expression affected NPC cell morphology and the expression of EMT markers in vitro. Furthermore, overexpression of EBNA1 inhibited the expression of microRNA 200a (miR-200a) and miR-200b and, in turn, up-regulated expression of their target genes, zinc finger E-box binding homeobox 1 ( ZEB1) and ZEB2, which are well known mediators of EMT. In addition, EBNA1-regulated miR-200a and miR-200b expression was mediated by transforming growth factor-1. CONCLUSIONSThe current findings provided novel insight into the vital role of EBNA1 in manipulating a molecular switch of EMT in EBV-positive NPC cells. Cancer 2014;120:363-372. (c) 2013 American Cancer Society.
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