Journal
JOURNAL OF VIROLOGY
Volume 81, Issue 1, Pages 166-172Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01953-06
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI052014] Funding Source: NIH RePORTER
- NIAID NIH HHS [R01 AI052014, AI52014] Funding Source: Medline
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Preintegration complexes (PICs) mediate retroviral integration, and recent results indicate an important role for the inner nuclear membrane protein emerin in orienting human immunodeficiency virus type 1 (HfV-1) PICs to chromatin for integration. Two other host cell proteins, the barrier-to-autointegration factor (BAF) and lamina-associated polypeptide 2 alpha (LAP2 alpha), seemed to play a similar preintegrative role for Moloney murine leukemia virus (MMLV) in addition to HIV-1. In contrast, we determined efficient HfV-1 and MMLV infection of HeLa-P4 cells following potent down-regulation of emerin, BAF, or LAP2 alpha protein by using short interfering RNA. Mouse embryo fibroblasts ablated for emerin protein through gene knockout support the same level of HIV-1 infection as cells derived from wild-type littermate control animals. As the expression of human emerin in mouse knockout cells fails to affect the level of infectivity achieved in its absence, we conclude that HIV-1 efficiently infects cells in the absence of emerin protein and, by extension, that emerin is not a universally important regulator of HIV-1 infectivity.
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