Journal
JOURNAL OF BACTERIOLOGY
Volume 189, Issue 8, Pages 3306-3311Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00018-07
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Funding
- Medical Research Council [G0300662B] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BB/D522746/1] Funding Source: Medline
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The role of DNA repair by nonhomologous-end joining (NHEJ) in spore resistance to UV, ionizing radiation, and ultrahigh vacuum was studied in wild-type and DNA repair mutants (recA, splB,ykoU, ykoU, and ykoU ykoV mutants) of Bacillus subtilis. NHEJ-defective spores with mutations in ykoU, ykoV, and ykoU ykoV were significantly more sensitive to UV, ionizing radiation, and ultrahigh vacuum than wild-type spores, indicating that NHEJ provides an important pathway during spore germination for repair of DNA double-strand breaks.
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